Abstract: This study evaluated the antibacterial, antifungal, and antioxidant effect of 7-hydroxy-4’,6-dimethoxy-isoflavone and essential oil of Myroxylon peruiferum. The compound was isolated and its structure elucidated by NMR. The chemical composition of essential oil determined by GC-MS analysis. To evaluation of antimicrobial activity, the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC) were performed. In addition to analysis of antioxidant activity, DPPH radical scavenging tests, iron chelating assay (FIC), antioxidant reducing power assay (FRAP) and β-carotene bleaching assay (BCB) were performed. For the essential oil were identified 24 organized compounds having as main constituents; Germacrene D (17.2%), α-pinene (14.8%) and E-caryophyllene (10.8%). The results showed that isoflavone (2000 to 156 μg/mL) and essential oil (5.0 to 1.25%) present antibacterial and antifungal activity against Gram-positive bacteria and filamentous fungi. The isoflavone and the essential oil also presented antioxidant activity in all the tests, mainly on inhibition of the oxidation of β-carotene test concentrations ranging from 60 to 100%. In conclusion, isoflavone and essential oil from M. peruiferum present an antimicrobial alternative against Gram-positive bacteria, especially of the genus Staphylococcus and dermatophyte fungi of the genus Trichophyton, as well as a natural compound antioxidant.

In this work, the allantoin was encapsulated with galactomannan extruded from Delonyx regia, by the spray drying technique. The microparticles obtained were structurally characterized by infrared spectroscopy (IR), thermal analysis, scanning electron microscopy (SEM) and the controlled release profile was evaluated. The results of IR suggested the formation of the polymeric wall of the microcapsules and SEM revealed spherical particles with an average size range of 2.84 ± 0.41 µm. X-ray diffraction showed the loss of crystallinity of the active after encapsulation and differential explanatory calorimetry exhibited the loss of its peak melt. The encapsulation efficiency for the microparticles was approximately 84% for the different environmental conditions. The coating of allantoin with the biopolymer promoted a gradual release of the drug over a period of 6 hours and these results are in accordance with the Brazilian Pharmacopoeia. The set of results obtained in this work is relevant and accredits the system developed as a possible alternative for the formation of encapsulated natural drugs destined to possible treatments of cutaneous cellular regeneration.

Dois novos furanoflavonoides, 5-hidróxi-2’’-isopropenil-3-metoxifurano-(2",3":7,8)-flavona e 5-hidróxi-2’’-(1-hidróxi-1-metiletil)-3-metoxifurano-(2",3":7,8)-flavona, juntamente com treze compostos conhecidos, foram isolados das cascas das raízes de Lonchocarpus obtusos (Leguminosae). As estruturas de todos os compostos isolados foram determinadas usando métodos espectrométricos tais como RMN 1D e 2D (COSY, HSQC e HMBC) e EMAR, além da comparação com dados espectrais descritos na literatura para compostos de estruturas semelhantes.

Two new furanoflavonoids, 5-hydroxy-2’’-isopropenyl-3-methoxyfurane-(2",3":7,8)-flavone and 5-hydroxy-2’’-(1-hydroxy-1-methylethyl)-3-methoxyfurane-(2",3":7,8)-flavone, along with thirteen other known compounds, were isolated from the root barks of Lonchocarpus obtusus (Leguminosae). The structures of all compounds were determined by spectrometric methods such as 1D and 2D NMR (COSY, HSQC and HMBC) and HREISMS beside comparison with spectral data for similar compounds.

A investigação fitoquímica da resina de Amburana cearensis A. C. Smith permitiu o isolamento de um novo composto: 3',4'-dimetóxi-1'-(7-metóxi-4-oxo-4H-cromen-3-il)benzo-2',5'-quinona (1), juntamente com seis compostos conhecidos e identificados como: 4,2',4'-triidroxichalcona (2), 7,8,3',4'-tetrametoxiisoflavona (3), 4,2',4'-triidroxi-3-metoxichalcona (4), 3,4,5-trimetoxicinamaldeido (5), 3',4'-dimetoxi-7-hidroxiisoflavona (6) e 6,7,4'-trimetoxi-3'-hidroxiisoflavona (7). As estruturas foram estabelecidas com base na análise dos dados espectrométricos de: IV, EI-EM, HR-ESI-EM e RMN incluindo experimentos 2D.

Phytochemical investigation of the resin of Amburana cearensis A. C. Smith allowed the isolation of a new compound: 3',4'-dimethoxy-1'-(7-methoxy-4-oxo-4H-cromen-3-yl)benzo-2',5'-quinone (1), together with six known compounds identified as: 4,2',4'-trihydroxychalcone (2), 7,8,3',4'-tetramethoxyisoflavone (3), 4,2',4'-trihydroxy-3-methoxychalcone (4), 3,4,5-trimethoxycinnamaldehyde (5), 3',4'-dimethoxy-7-hydroxyisoflavone (6) and 6,7,4'-trimethoxy-3'-hydroxyisoflavone (7). The structures were established from the IR, HR-ESI-MS and NMR spectral data, including 2D-NMR experiments.

Este trabalho descreve a composição química dos óleos essenciais e o isolamento de onze substâncias de Eupatorium ballotifolium Kunth, Asteraceae. Os óleos essenciais foram obtidos por hidrodestilação, analisados por CG-EM e avaliados quanto às suas atividades frente à enzima acetilcolinesterase. O rendimento dos óleos foi de 0,11% para as folhas e 0,03% para os talos. Os principais constituintes dos óleos foram os sesquiterpenos β-cariofileno (24,9 e 22,2%), espatulenol (17,7 e 12,4%) e epóxi-allo-aromadendreno (23,0 e 23,6%). Do extrato hexânico da parte aérea foi isolada a mistura de β-sitosterol e estigmasterol, incluindo suas formas glicosiladas, e os triterpenos acetato de taraxasterila e taraxasterol, enquanto, do extrato etanólico foram isolados os flavonóides nepetina and 3-O-glicosil-quercetina. Do extrato hexânico das raízes foram isolados os triterpenos epi-friedelanol e damara-20,24-dien-3β-ol e do extrato etanólico a cumarina 11-hidroxi-11,12-di-hidroobliquina. As estruturas de todos os compostos foram determinadas usando técnicas espectroscópica tais como IV, EM e RMN ¹H e 13C.

This work describes the chemical composition of the essential oils and the isolation of eleven substances from Eupatorium ballotifolium Kunth, Asteraceae. The essential oils were obtained by hydrodistillation, analyzed by GC/MS and evaluated towards the acetylcholinesterase enzyme. The oils yield was of 0.11% for the leaves and 0.03% for the stems. The main constituents of the oils were the sesquiterpenes β-caryophyllene (24.9 and 22.2%), spathulenol (17.7 and 12.4%) and epoxy-allo-aromadendrene (23.0 and 23.6%). From the hexane extract of the aerial part were isolated a mixture of sitosterol and stigmasterol, its glucosides, and the triterpenes taraxasteryl acetate and taraxasterol, while from the ethanol extract were obtained the flavonoids nepetin and 3-O-glucoside-quercetin. The triterpenes epi-friedelanol and dammara-20,24-dien-3β-ol were obtained from the hexane extract of roots, while the coumarin 11-hydroxy-11,12-di-hydroobliquine was obtained from the ethanol extract. The structures of all compounds were determinate based on spectroscopic methods such as IR, MS and ¹H and 13C NMR

Croton zehntneri Pax. & K. Hoffm. (Euphorbiaceae) is an aromatic plant native of the northeaster region of Brazil; and is popularly known as "canela de cunhã". The chemical composition of the essential oils from leaves, stalks and roots from three chemotypes of C. zehntneri obtained by hydrodistillation were analyzes by GC-MS. E-anethole, was the main component in the essential oils of all plants parts of che-motype 1. While the phenylpropanoids stragole, eugenol, Z-methyl isoeugenol and E-methyl isoeugenol were the major component in the essential oils of chemotypes 2 and 3, respectively. On TLC the essential oils and the major compounds showed an acetylcholine esterase inhibitory effect.

Croton zehntneri Pax. & K. Hoffm. (Euphorbiaceae) es una planta aromática nativa de la región nororiental de Brasil, y es popularmente conocido como "canela de cunhã". La composición química de los aceites esenciales de hojas, tallos y raíces de três quimiotipos de C. zehntneri obtenido mediante hidrodestilación fueron examinados por GC-MS. E-anetol, fue principal componente en los aceites esenciales de todas las partes de la planta de quimiotipo 1. Si bien la fenilpropanóides estragol, eugenol, Z-metil isoeugenol and E-metil isoeugenol fueron el componente principal en los aceites esenciales de quimiotipos 2 e 3, respectivamente. El TLC estos aceites esenciales y los principales compuestos mostraron una acetilcolina esterase efecto inhibitorio.

Usando reações clássicas como esterificação e oxidação, uma série de derivados foi obtida a partir da mistura alfa- e beta-amirina, constituintes majoritários da resina de Protium heptaphyllum. Os compostos obtidos foram caracterizados por dados espectroscópicos como: IV, RMN de ¹H e de 13C e comparação com dados da literatura.

Using classic reactions such as esterification and oxidation, a series of derivatives was obtained from the alpha- and beta-amyrin mixture, major compounds of the Protium heptaphyllum resin. The obtained compounds were characterized by spectroscopic data such as: IR, ¹ H and 13C NMR and comparison with literature.